Recombinant MUC-1 Antibody
This mAb reacts with MUC1, a large transmembrane glycoprotein expressed on the ductal surface of normal glandular epithelia. The dominant epitope of this MAb involves both amino acids as well as sugar moieties. Neuraminidase treatment destroys the antigen. It is a very good tracer agent in CA15.3 assays. The extracellular domain of MUC1 largely consists of a highly conserved, O-glycosylated 20 amino acids tandem repeat which can occur 30-100 times per molecule depending on the length of the allele involved. In the vast majority of human carcinomas this protein is up-regulated and poorly glycosylated and appears on the cell surface in a non-polarized fashion.
Product Specifications
CAS Number
9000-83-3
Specifications
Immunohistochemistry (FFPE only; not suitable for frozen tissues) : 1-2 µg/mL for 30 min at RT, Western blot: 1-2 µg/mL
UniProt
P15941
Host
Mouse
Reactivity
Human
Immunogen
Human milk fat globule membranes were used as the immunogen for this recombinant MUC-1 antibody.
Clonality
Recombinant Monoclonal
Isotype
IgG2b κ
Clone
RMUC1/960
Type
Recombinant
Applications
IHC-P, WB
Purity
Protein G affinity chromatography
Format
Purified
Buffer
0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide
Reconstitution
Store the recombinant MUC-1 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).
Limitations
This recombinant MUC-1 antibody is available for research use only.
Storage Conditions
Store the recombinant MUC-1 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .
Formulation
0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05% sodium azide
Applications Notes
Location
Cytoplasmic and cell surface
Image Legend
Frequently Asked Questions
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