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E. coli Antibody

This mAb shows specificity to E. coli in a simple ELISA. Escherichia coli are Gram negative bacterium that are commonly found in the lower intestine of warm-blooded organisms (endotherms) . Their serological types are determined in combination with somatic antigens (O group: O1-O173) and flagella antigens (H type: H1-H56) . The E. coli that cause intestinal infectious diseases including diarrhea, acute gastritis or colitis are referred to as pathogenic E. coli, which are classified into the following four groups according to differences in the mode of pathogenicity; enteropathogenic E. coli (EPEC), enteroinvasive E. coli (EIEC), enterotoxigenic E. coli (ETEC) and enterohemorrhagic E. coli (EHEC) . Although the identification of pathogenic E. coli requires verification of their pathogenicity, pathogenic E. coli often have specific serotypes; therefore, typing of the serogroup and serotype is necessary in screening pathogenic E. coli.

Product Specifications

CAS Number

9007-83-4

Specifications

ELISA: 1-5 µg/mL for coating (order BSA/sodium azide-free format), Immunofluorescence: 1-2 µg/mL

Host

Mouse

Reactivity

E. coli

Immunogen

A crude sonicate of E. coli was used as the immunogen for the E. coli antibody.

Clonality

Monoclonal

Isotype

IgM k

Clone

9.88

Applications

ELISA, IF

Purity

PEG precipitation followed by dialysis in 1X PBS

Format

Purified

Buffer

0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide

Reconstitution

Store the E. coli O157 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Limitations

This E coli O157 antibody is available for research use only.

Storage Conditions

Store the E. coli antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .

Formulation

0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05% sodium azide

Applications Notes

Optimal dilution of the E. coli antibody should be determined by the researcher.

Location

Whole organism

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