Desmin Antibody

Cytoskeletal intermediate filaments (IFs) constitute a diverse group of proteins that are expressed in a highly tissue-specific manner. IFs are constructed from two-chain α -helical coiled-coil molecules arranged on an imperfect helical lattice, and have been widely used as markers for distinguishing individual cell types within a tissue and identifying the origins of metastatic tumors. Vimentin is an IF general marker of cells originating in the mesenchyme. Vimentin and Desmin, a related class III IF, are both expressed during skeletal muscle development. Desmin, a 469 amino acid protein found near the Z line in sarcomeres, is expressed more frequently in adult differentiated state tissues. Anti-desmin detects cells of normal smooth, skeletal, and cardiac muscles. Antibody reacts with leiomyomas, leiomyosarcoma, rhabdomyomas, rhabdomyosarcoma, and perivascular cells of glomus tumors of the skin.

Product Specifications

CAS Number

9007-83-4

Specifications

Immunofluorescence: 1-2 µg/mL, Flow cytometry: 0.5-1ug/10^6 cells, Western blot: 0.5-1 µg/mL, Immunohistochemistry (FFPE) : 1-2 µg/mL for 30 min at RT

UniProt

P17661

Host

Mouse

Reactivity

Human, Mouse, Rat

Immunogen

Recombinant human Desmin was used as the immunogen for the Desmin antibody.

Clonality

Monoclonal

Isotype

IgG1 k

Clone

DES/1711

Applications

IF, FACS, WB, IHC-P

Purity

Protein G affinity chromatography

Format

Purified

Buffer

0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide

Reconstitution

Store the Desmin antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Limitations

This Desmin antibody is available for research use only.

Storage Conditions

Store the Desmin antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .

Formulation

0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05% sodium azide

Applications Notes

Optimal dilution of the Desmin antibody should be determined by the researcher.

Location

Cytoplasmic

Image Legend

IHC testing of FFPE human uterus with Desmin antibody (clone DES/1711) . Required HIER: boil tissue sections in 10mM citrate buffer, pH 6, for 10-20 min.