CD31 Antibody / PECAM-1
CD31 (PECAM-1) is a transmembrane glycoprotein member of the immunoglobulin supergene family of adhesion molecules. CD31 is expressed by stem cells of the hematopoietic system and is primarily used to identify and concentrate these cells for experimental studies as well as for bone marrow transplantation. Anti-CD31 has shown to be highly specific and sensitive for vascular endothelial cells. Staining of nonvascular tumors (excluding hematopoietic neoplasms) is rare. CD31 MAb reacts with normal, benign, and malignant endothelial cells which make up blood vessel lining. The level of CD31 expression can help to determine the degree of tumor angiogenesis, and a high level of CD31 expression may imply a rapidly growing tumor and potentially a predictor of tumor recurrence.
Product Specifications
CAS Number
9007-83-4
Specifications
Flow cytometry: 5ul per test per one 10^6 cells in 0.1ml or 5ul per 100ul of whole blood, Immunofluorescence: 1:50-1:100
UniProt
P16284
Host
Mouse
Reactivity
Human
Immunogen
A membrane preparation of a spleen from a patient with hairy cell leukemia was used as the immunogen for this CD31 antibody.
Clonality
Monoclonal
Isotype
IgG1 k
Clone
JC/70A
Applications
FACS, IF
Purity
Protein G affinity chromatography
Format
CF488 Conjugate
Buffer
500 ul at 0.1 mg/ml with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide
Reconstitution
Store the CD31 antibody at 2-8oC, protected from light.
Limitations
This CD31 antibody is available for research use only.
Storage Conditions
Store the CD31 antibody at 2-8°C, protected from light.
Product Datasheet
https://www.nsjbio.com/tds-pdf/cd31-antibody-pecam-1-jc70a-v3143cf488
Formulation
500 ul at 0.1 mg/mL with 0.1 mg/mL BSA (US sourced), 0.05% sodium azide
Applications Notes
Optimal dilution of the CD31 antibody should be determined by the researcher.
Location
Cell surface, cytoplasmic
Image Legend
Flow cytometry testing of human Jurkat cells. Black: cells alone; Grey: isotype control; Green: CD31 antibody (clone JC/70A) .
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