LRWD1 Antibody (N-term) [APR30848G]

Product Specifications

Background

Tissue Location: Testis-specific. Drastically down-regulated in testis from patients with Sertoli cell-only syndrome (SCOS)

Overview

We constantly strive to ensure we provide our customers with the best antibodies. As a result of this work we offer this antibody in purified format. We are in the process of updating our datasheets. If you have any questions regarding this update, please feel free to contact our technical support team. This product is a high quality LRWD1 Antibody (N-term) [APR30848G].

Synonyms

Leucine-rich repeat and WD repeat-containing protein 1, Centromere protein 33, CENP-33, Origin recognition complex-associated protein, ORC-associated protein, ORCA, LRWD1, CENP33, ORCA

Gene Name

LRWD1

Gene ID

222229

UniProt

Q9UFC0

Chromosomal Location

Nucleus. Chromosome, centromere. Chromosome, telomere. Cytoplasm, cytoskeleton, microtubule organizing center, centrosome {ECO:0000250|UniProtKB:Q8BUI3}. Chromosome, centromere, kinetochore. Note=Localizes to heterochromatin during G1 phase. Restricted to centromeres or telomeres as cells progress though S phase. When cells enter mitosis, relocalizes to centromeres Recruitment to pericentric heterochromatin largely depends on the presence of H3K9me3

Applications

WB, IHC-P

Dilution

WB--1:1000 IHC-P--1:100~500

Form

Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.

Function

Required for G1/S transition. Recruits and stabilizes the origin recognition complex (ORC) onto chromatin during G1 to establish pre-replication complex (preRC) and to heterochromatic sites in post- replicated cells. Binds a combination of DNA and histone methylation repressive marks on heterochromatin. Binds histone H3 and H4 trimethylation marks H3K9me3, H3K27me3 and H4K20me3 in a cooperative manner with DNA methylation. Required for silencing of major satellite repeats. May be important ORC2, ORC3 and ORC4 stability.