C-Kit Antibody
This mAb recognizes a protein of 145kDa, identified as CD117/p145kit/c-Kit. It is found on a wide variety of tumor cells including follicular and papillary carcinoma of thyroid, adenocarcinomas from endometrium, lung, ovary, pancreas, and breast as well as malignant melanoma, endodermal sinus tumor, and small cell carcinoma. However, anti-CD117/c-Kit has been particularly useful in differentiating gastrointestinal stromal tumors from Kaposi's sarcoma, tumors of smooth muscle origin, fibromatosis, and neural tumors of the GI tract. Anti-CD117/c-Kit is also useful in recognizing myeloblasts in bone marrow biopsy and clot section.
Product Specifications
Specifications
Western blot: 0.5-1 µg/mL for 2 hours at RT
UniProt
P10721
Host
Mouse
Reactivity
Human
Immunogen
A recombinant human fragment around amino acids 100-300 was used as the immunogen for the c-Kit antibody.
Clonality
Monoclonal
Isotype
IgG1 k
Clone
KIT/982
Applications
WB
Purity
Protein G affinity chromatography
Format
Purified
Buffer
0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide
Reconstitution
Store the c-Kit antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).
Limitations
This c-Kit antibody is available for research use only.
Storage Conditions
Store the c-Kit antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .
Product Datasheet
https://www.nsjbio.com/tds-pdf/c-kit-antibody-kit982-v2648
Formulation
0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05% sodium azide
Applications Notes
Optimal dilution of the c-Kit antibody should be determined by the researcher.
CAS Number
9007-83-4
Location
Cell surface and cytoplasmic
Image Legend
Western blot of a recombinant protein fragment with c-Kit antibody (KIT/982) .
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