ICAM-3 Antibody
Recognizes an N-glycosylated glycoprotein of 120kDa with intra-chain disulfide bonds, identified as CD50 or ICAM-3 (WS: IV & V) . Its epitope localizes in the D2 extracellular domain and is resistant to neuraminidase and proteases. CD50 is the major ligand for LFA-1 (CD11a/CD18) and may have signalling role to increase adhesion. It is expressed on thymocytes and T lymphocytes and is resistant to treatment with phosphatidylinositol (PI) phospholipase C. This mAb inhibits primary mixed lymphocyte culture (MLC) but not secondary MLC, cytotoxicity or proliferation induced by mitogens. It blocks binding of NK1-L16 stimulated T cells to L cells expressing CD50. This mAb is excellent for staining of formalin/paraffin tissues.
Product Specifications
CAS Number
9007-83-4
Specifications
Flow cytometry: 0.5-1ug/10^6 cells, Immunofluorescence: 0.5-1 µg/mL, Immunohistochemistry (FFPE) : 0.5-1 µg/mL for 30 min at RT
UniProt
P32942
Host
Mouse
Reactivity
Human
Immunogen
Stimulated human leukocytes were used as the immunogen for the ICAM-3 antibody.
Clonality
Monoclonal
Isotype
IgG2a κ
Clone
101-1D2
Applications
FACS, IF, IHC-P
Purity
Protein G affinity chromatography
Format
Purified
Buffer
1 mg/ml in 1X PBS; BSA free, sodium azide free
Reconstitution
Store the ICAM-3 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).
Limitations
This ICAM-3 antibody is available for research use only.
Storage Conditions
Store the ICAM-3 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .
Product Datasheet
https://www.nsjbio.com/tds-pdf/icam-3-antibody-101-1d2-v2607
Formulation
1 mg/mL in 1X PBS; BSA free, sodium azide free
Applications Notes
Location
Membrane, cytoplasm
Image Legend
SDS-PAGE Analysis of Purified, BSA-Free ICAM-3 Antibody (clone 101-1D2) . Confirmation of Integrity and Purity of the Antibody.
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