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Anti-CD14 Antibody

CD14 is anchored to cells by linkage to glycosylphosphatidylinositol (GPI) and functions as a high affinity receptor for complexes of LPS and LPS binding protein (LBP) . Soluble CD14, also binding to LPS, acts at physiological concentration as an LPS agonist and has, at higher concentrations, an LPS antagonizing effect in cell activation.

Product Specifications

CAS Number

9007-83-4

Specifications

Flow cytometry: 1-2ug/10^6 cells, Immunofluorescence: 1-2 µg/mL

UniProt

P08571

Host

Mouse

Reactivity

Human

Immunogen

Full length human recombinant CD14 protein was used as the antigen for this anti-CD14 antibody.

Clonality

Monoclonal

Isotype

IgG1 k

Clone

LPSR/553

Applications

FACS, IF

Purity

Protein G affinity chromatography

Format

Purified

Buffer

1X PBS, pH 7.4

Reconstitution

Store the anti-CD14 antibody (with azide) at 2-8oC. The azide-free format should be aliquoted and stored at -20oC or colder.

Limitations

This anti-CD14 antibody is available for research use only.

Storage Conditions

Store the anti-CD14 antibody (with azide) at 2-8°C. The azide-free format should be aliquoted and stored at -20°C or colder.

Formulation

0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05% sodium azide

Applications Notes

The stated application concentrations are suggested starting amounts. Variations in protocols, secondaries and substrates may require the anti-CD14 antibody to be titered up or down for optimal performance.

Location

Cell surface, Secreted, Cytoplasmic (Golgi)

Image Legend

SDS-PAGE Analysis of Purified, BSA-Free Anti-CD14 Antibody (clone LPSR/553) . Confirmation of Integrity and Purity of the Antibody.

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