CD45RO Antibody
Recognizes a 180-185kDa protein, identified as isoform of leukocyte common antigen (CD45RO) . This antibody reacts with mature activated T-cells, most thymocytes, and a sub-population of resting T-cells within both CD4 and CD8 subsets. It shows no reactivity with normal B or natural killer cells, but reacts with granulocytes and monocytes. Reportedly, it is useful to identify T-cell lymphomas and leukemias. It rarely stains NK cells or B-cell lymphomas.
Product Specifications
CAS Number
9007-83-4
Specifications
Flow cytometry: 1-2ug/10^6 cells, Immunofluorescence: 1-2 µg/mL
UniProt
P08575
Host
Mouse
Reactivity
Human
Immunogen
Lentillectin purified glycoproteins obtained from HuPBL and from a CLL (B cell) were used as immunogen for this CD45RO antibody.
Clonality
Monoclonal
Isotype
IgG2a κ
Clone
190-2F2.5
Applications
FACS, IF
Purity
Protein G affinity chromatography
Format
Purified
Buffer
1X PBS, pH 7.4
Reconstitution
The CD45RO antibody (with azide) can be stored at 2-8oC. The azide-free format should be aliquoted and stored at -20oC or colder.
Limitations
This CD45RO antibody is available for research use only.
Storage Conditions
The CD45RO antibody (with azide) can be stored at 2-8°C. The azide-free format should be aliquoted and stored at -20°C or colder.
Formulation
1 mg/mL in 1X PBS; BSA free, sodium azide free
Applications Notes
Due to variation in protocol and secondary antibody used, the CD45RO antibody may need to be titered for optimal performance.
Location
Cell surface
Image Legend
Immunofluorescent staining of PFA-fixed human Jurkat cells with CD45RO antibody (clone 190-2F2.5, green) and Reddot nuclear stain (red) .
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