ACTH Antibody / Synacthen

POMC (pro-opiomelanocortin or corticotropin-lipotropin) is a 267 amino acid polypeptide hormone precursor that goes through extensive, tissue-specific post-translational processing by convertases. POMC is cleaved into ten hormone chains named NPP, ACTH, alpha-MSH (Melanocyte Stimulating Hormone), beta-MSH, gamma-MSH, CLIP (corticotropin-like intermediary peptide), Lipotropin-beta, Lipotropin-gamma, beta-endorphin and Met-enkephalin. ACTH (also called Corticotropin) is a 39 amino acid active peptide produced by the anterior pituitary. Clone AH26 antibody is specific to Synacthen (aa 1-24 of ACTH) and does not react with CLIP (aa 17-39) . ACTH is produced by cells of immune system (T-cells, B-cells, and macrophages) in response to stimuli associated with stress. Specific antibody is a useful marker in classification of pituitary tumors and the study of pituitary disease. It reacts with ACTH-producing cells (corticotrophs) . It also may react with other tumors (e.g. some small cell carcinomas of the lung) that cause paraneoplastic syndromes by secreting ACTH.

Product Specifications

CAS Number

9007-83-4

Specifications

Immunohistochemistry (FFPE) : 0.5-1 µg/mL for 30 min at RT

Host

Mouse

Reactivity

Human, Mouse, Rat

Immunogen

A synthetic peptide corresponding to aa 1-24 of human ACTH was used as the immunogen for this ACTH antibody.

Clonality

Monoclonal

Isotype

IgG1 k

Clone

AH26

Applications

IHC-P

Purity

Protein G affinity chromatography

Format

Purified

Buffer

1X PBS, pH 7.4

Reconstitution

Store the ACTH antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Limitations

This ACTH antibody is available for research use only.

Storage Conditions

Store the ACTH antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .

Formulation

0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05% sodium azide

Applications Notes

The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the ACTH antibody to be titered up or down for optimal performance.

Location

Cytoplasmic

Image Legend

IHC testing of FFPE human pituitary gland stained with ACTH antibody (clone AH26) . HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.