CD11a Antibody / LFA-1

This antibody recognizes a protein of 180kDa, identified as CD11a. CD11a complexes with the beta 2 subunit of the integrin family, CD18, to form the cell surface heterodimer called LFA-1 or CD11a/CD18. LFA-1 is expressed on all leukocytes including lymphocytes, monocytes, and granulocytes. It is involved in leukocyte adhesion to its ligands including intercellular adhesion molecule-1 (ICAM-1 or CD54), ICAM-2 (CD102), ICAM-3 (CD50) and Telencephalin (TLN) and play a role in most immune/inflammatory responses.

Product Specifications

CAS Number

9007-83-4

Specifications

Flow cytometry: 1-2ug/10^6 cells, Immunofluorescence: 1-2 µg/mL Functional studies (order BSA/sodium azide-free format)

Host

Mouse

Reactivity

Human

Immunogen

Stimulated human leukocytes were used as the immunogen for this CD11b antibody.

Clonality

Monoclonal

Isotype

IgG2b κ

Clone

CRIS-3

Applications

FACS, IF Functional studies

Purity

Protein G affinity chromatography

Format

Purified

Buffer

1X PBS, pH 7.4

Reconstitution

Store the CD11a antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Limitations

This CD11a antibody is available for research use only.

Storage Conditions

Store the CD11a antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .

Formulation

0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05% sodium azide

Applications Notes

The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the antibody to be titered up or down for optimal performance.