USP44 Antibody
Ubiquitin carboxyl-terminal hydrolase 44 is an enzyme that in humans is encoded by the USP44 gene. It is mapped to 12q22. The protein encoded by this gene is a protease that functions as a deubiquitinating enzyme. The encoded protein is thought to help regulate the spindle assembly checkpoint by preventing early anaphase onset. This protein specifically deubiquitinates CDC20, which stabilizes the anaphase promoting complex/cyclosome.
Product Specifications
CAS Number
9007-83-4
Specifications
Western blot: 0.5-1 µg/mL, Immunohistochemistry: 1-2 µg/mL, Immunofluorescence: 2-4 µg/mL, Flow cytometry: 2-4 µg/mL, Direct ELISA: 0.1-0.5 µg/mL
UniProt
Q9H0E7
Host
Rabbit
Reactivity
Human, Mouse, Rat
Immunogen
Recombinant human protein (amino acids R211-S712) was used as the immunogen for the USP44 antibody.
Clonality
Polyclonal
Isotype
IgG
Applications
WB, IHC-P, IF, FACS, Direct ELISA
Purity
Affinity purified
Format
Antigen affinity purified
Buffer
Lyophilized from 1X PBS with 2% Trehalose and 0.025% sodium azide
Reconstitution
Limitations
This USP44 antibody is available for research use only.
Storage Conditions
Formulation
0.5 mg/mL if reconstituted with 0.2ml sterile DI water
Applications Notes
Optimal dilution of the USP44 antibody should be determined by the researcher.
Location
Nuclear
Image Legend
IHC staining of FFPE human testis cancer with USP44 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
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