BiP Antibody / GRP78 / HSPA5

The protein encoded by the HSPA5 gene is a member of the heat shock protein 70 (HSP70) family. It is localized in the lumen of the endoplasmic reticulum (ER), and is involved in the folding and assembly of proteins in the ER. As this protein interacts with many ER proteins, it may play a key role in monitoring protein transport through the cell. [RefSeq]

Product Specifications

CAS Number

9000-83-3

Specifications

Western blot: 1:500-1:2000, Immunohistochemistry (FFPE) : 1:50-1:200, Immunofluorescence: 1:50-1:100

UniProt

P11021

Host

Rabbit

Reactivity

Human, Mouse, Rat

Immunogen

A synthetic peptide specific to human HSPA5 / BiP was used as the immunogen for the BiP antibody.

Clonality

Monoclonal

Isotype

IgG

Clone

BOO-8

Type

Recombinant

Applications

IHC-P, WB, IF

Purity

Affinity purified

Format

Purified

Buffer

Antibody in PBS with 0.02% sodium azide, 50% glycerol and 0.4-0.5mg/ml BSA

Reconstitution

Store the BiP antibody at -20oC.

Limitations

This BiP antibody is available for research use only.

Storage Conditions

Store the BiP antibody at -20°C.

Formulation

Antibody in PBS with 0.02% sodium azide, 50% glycerol and 0.4-0.5 mg/mL BSA

Applications Notes

Optimal dilution of the BiP antibody should be determined by the researcher.

Location

Cytoplasmic

Image Legend

IHC staining of FFPE human pancreas tissue with BiP antibody. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min followed by cooling at RT for 20 min.