Adducin α/β (phospho-S726/713) polyclonal antibody

Product Specifications

Background

Adducins are a family of cytoskeletal proteins encoded by three genes (alpha, beta, gamma) . Adducin is a heterodimeric protein that consists of related subunits, which are produced from distinct genes but share a similar structure. Alpha and beta adducin include a protease-resistant N-terminal region and a protease-sensitive, hydrophilic C-terminal region. Alpha and gamma adducins are ubiquitously expressed. In contrast, beta adducin is expressed at high levels in brain and hematopoietic tissues. Adducin binds with high affinity to Ca2+/calmodulin and is a substrate for protein kinases A and C. Alternative splicing results in multiple variants encoding distinct isoforms; however, not all variants have been fully described.

CAS Number

9007-83-4

Structure Composition

Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Product Name Alternative

Alpha-adducin; Erythrocyte adducin subunit alpha; ADD1; ADDA; α-Adducin; Adducin1; Adducin-1; α Adducin; Adducin-α; Beta-adducin; Erythrocyte adducin subunit beta; ADD2; ADDB; β-Adducin; Adducin1; Adducin-1; β Adducin; Adducin β; Adducin-β

Swiss Prot

P35611/P35612

Reactivity

Human, Mouse, Rat

Immunogen

Synthetic phosphopeptide derived from human Adducin α around the phosphorylation site of Serine 726.

Conjugation

Unconjugated

Applications

WB IHC

Dilution

WB: 1:500~1:1000 IHC: 1:50~1:200

Purity

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .

Modification

Phosphorylation

Molecular Weight

~ 81 kDa

Storage Conditions

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Notes

For research use only, not for use in diagnostic procedure.

Specificity

P-Adducin α/β (S726/713) polyclonal antibody detects endogenous levels of Adducin α protein only when phosphorylated at Ser726 and Adducin β protein only when phosphorylated at Ser713.

Applications Notes

Western blot (WB) analysis of p-Adducin α/β (S726/713) polyclonal antibody at 1:500 dilution Lane1:Hela cell lysate treated with PMA Lane2:SP2/0 cell lysate treated with PMA Lane3:PC12 cell lysate treated with PMA