CTBP1 Antibody

CTBP1, C-terminal-binding protein 1, is a protein that in humans is encoded by the CTBP1 gene. The CtBP1 protein binds to the C-terminus of adenovirus E1A proteins. This gene is mapped to 4p16.This phosphoprotein is a transcriptional repressor (corepressor) and may play a role during cellular proliferation. This protein and the product of a second closely related gene, CTBP2, can dimerize. CtBP1 and CtBP2 preferentially associate with the E1A via a 5 amino acid motif, PLDLS, to repress E1A induced oncogenesis and cellular transformation. CtBP1 is expressed from embryo to adult, but CtBP2 is mainly expressed during embryogenesis. During skeletal and T cell development, CtBP1 and CtBP2 associate with the PLDLSL domain of ?EF1, a cellular zinc finger-homeodomain protein, and thereby enhances ?EF1-induced transcriptional silencing. In addition, CtBP complexes with CtIP, a 125 kDa protein that recognizes distinctly different protein motifs from CtBP. CtIP binds to the BRCT repeats within the breast cancer gene BRCA1 and enables CtBP to influence BRCA1 activity. Both proteins can also interact with a polycomb group protein complex which participates in regulation of gene expression during development. Alternative splicing of transcripts from this gene results in multiple transcript variants.

Product Specifications

CAS Number

9007-83-4

Specifications

Western blot: 0.1-0.5 µg/mL, Immunohistochemistry (FFPE) : 1-2 µg/mL, Immunofluorescence (FFPE) : 2-5 µg/mL, Flow cytometry: 1-3ug/million cells

UniProt

Q13363

Host

Rabbit

Reactivity

Human, Mouse, Rat

Immunogen

Amino acids HGLPPVAHPPHAPSPGQTVKPEADRDHASDQL of human CTBP1 were used as the immunogen for the CTBP1 antibody.

Clonality

Polyclonal

Isotype

IgG

Applications

WB, IHC-P, IF, FACS

Purity

Antigen affinity

Format

Antigen affinity purified

Buffer

Lyophilized from 1X PBS with 2.5% BSA and 0.025% sodium azide

Reconstitution

After reconstitution, the CTBP1 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Limitations

This CTBP1 antibody is available for research use only.

Storage Conditions

After reconstitution, the CTBP1 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Formulation

0.5 mg/mL if reconstituted with 0.2ml sterile DI water

Applications Notes

Optimal dilution of the CTBP1 antibody should be determined by the researcher.

Location

Nuclear, cytoplasmic

Image Legend

Immunofluorescent staining of FFPE human T-47D cells with CTBP1 antibody (red) and Alpha Tubulin mAb (green) . HIER: steam section in pH6 citrate buffer for 20 min.