HSP60 Antibody / HSPD1

NFKBTBETTLR2

Product Specifications

CAS Number

9007-83-4

Specifications

Western blot: 0.5-1 µg/mL, Immunohistochemistry (FFPE) : 0.5-1 µg/mL, Immunocytochemistry: 0.5-1 µg/mL, Immunofluorescence: 2-3 µg/mL, Flow cytometry: 1-3ug/10^6 cells

Host

Rabbit

Reactivity

Human, Mouse, Rat

Immunogen

Human partial recombinant protein (AA 260-496) was used as the immunogen for this HSP60 antibody.

Clonality

Polyclonal

Isotype

IgG

Applications

WB, IHC-P, ICC, IF, FACS

Purity

Antigen affinity

Format

Antigen affinity purified

Buffer

Lyophilized from 1X PBS with 2.5% BSA and 0.025% sodium azide

Reconstitution

After reconstitution, the HSP60 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Limitations

This HSP60 antibody is available for research use only.

Storage Conditions

After reconstitution, the HSP60 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Formulation

0.5 mg/mL if reconstituted with 0.2ml sterile DI water

Applications Notes

The stated application concentrations are suggested starting amounts. Titration of the HSP60 antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Image Legend

Western blot testing of HSP60 antibody and Lane 1: rat kidney; 2: mouse kidney; 3: (m) HEPA; 4: (r) NRK; 5: (r) PC12; 6: human SW620; 7: (h) A549; 8: (h) A431; 9: (h) HeLa lysate. Predicted/observed molecular weight: ~60 kDa.