HNF1A Antibody

HNF1 homeobox A (hepatocyte nuclear factor 1 homeobox A), also known as HNF1A or TCF1, is a human gene. It is mapped to chromosome 12q24.3. The protein encoded by this gene is a transcription factor that is highly expressed in the liver and is involved in the regulation of the expression of several liver-specific genes. HNF1A can function as a dimer. Deletion of the activation domains or interruption of the HNF1-binding sites in the LCT promoter resulted in complete loss of transcriptional activity.

Product Specifications

CAS Number

9007-83-4

Specifications

Western blot: 0.5-1 µg/mL

Host

Rabbit

Reactivity

Human

Immunogen

Human partial recombinant protein (AA 431-631) was used as the immunogen for this HNF1A antibody.

Clonality

Polyclonal

Isotype

IgG

Applications

Purity

Antigen affinity

Format

Antigen affinity purified

Buffer

Lyophilized from 1X PBS with 2.5% BSA and 0.025% sodium azide

Reconstitution

After reconstitution, the HNF1A antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Limitations

This HNF1A antibody is available for research use only.

Storage Conditions

After reconstitution, the HNF1A antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Formulation

0.5 mg/mL if reconstituted with 0.2ml sterile DI water

Applications Notes

The stated application concentrations are suggested starting amounts. Titration of the HNF1A antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Image Legend

Western blot testing of human 1) HeLa and 2) HepG2 cell lysate with HNF1A antibody. Predicted molecular weight ~67 kDa, commonly observed at 67-80 kDa.