PCSK9 Antibody

Proprotein convertase subtilisin/kexin type 9 is an enzyme that in humans is encoded by the PCSK9 gene. This gene encodes a proprotein convertase belonging to the proteinase K subfamily of the secretory subtilase family. By genomic sequence analysis, PCSK9 was mapped to chromosome 1p32. This gene is a crucial player in the regulation of plasma cholesterol homeostasis. It may prevent the recycling of LDLR from endosomes to the cell surface or direct it to lysosomes for degradation. It can induce ubiquitination of LDLR leading to its subsequent degradation. This gene is involved in the disposal of non-acetylated intermediates of BACE1 in the early secretory pathway.

Product Specifications

CAS Number

9007-83-4

Specifications

Western blot: 0.5-1 µg/mL, Immunohistochemistry (FFPE) : 2-5 µg/mL

UniProt

Q8NBP7

Host

Rabbit

Reactivity

Human

Immunogen

Amino acids 671-687 (AVTAVAICCRSRHLAQA-human) were used as the immunogen for this PCSK9 antibody.

Clonality

Polyclonal

Isotype

IgG

Applications

WB, IHC-P

Purity

Antigen affinity

Format

Antigen affinity purified

Buffer

Lyophilized from 1X PBS with 2% Trehalose

Reconstitution

After reconstitution, the PCSK9 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Limitations

This PCSK9 antibody is available for research use only.

Storage Conditions

After reconstitution, the PCSK9 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Formulation

0.5 mg/mL if reconstituted with 0.2ml sterile DI water

Applications Notes

The stated application concentrations are suggested starting points. Titration of the PCSK9 antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Location

Cell surface, cytoplasm, secreted

Image Legend

IHC staining of FFPE human liver cancer tissue with PCSK9 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.