NOX5 Antibody
NADPH Oxidase 5, also known as NOX5A or NOX5B, is a protein which in humans is encoded by the NOX5 gene. It is a NADPH oxidase that generates superoxide and functions as an H+ channel in a Ca2+-dependent manner. Banfi et al. (2001) found that, when heterologously expressed, NOX5 was quiescent in unstimulated cells. However, in response to elevations of the cytosolic Ca2+ concentration, it generated large amounts of superoxide. Using RT-PCR and Southern and Western blot analyses, Kamiguti et al. (2005) identified NOX5 as a flavin-containing Ca2+-dependent oxidase present in hairy leukemic cells (HC), but not normal B cells.
Product Specifications
CAS Number
9007-83-4
Specifications
Western blot: 0.5-1 µg/mL, IHC (FFPE) : 0.5-1 µg/mL, Immunocytochemistry: 0.5-1 µg/mL
UniProt
Q96PH1
Host
Rabbit
Reactivity
Human
Immunogen
An amino acid sequence from the C-terminus of human NADPH Oxidase 5 (KVLKGHCEKFGFRFFQENF) was used as the immunogen for this NOX5 antibody.
Clonality
Polyclonal
Isotype
IgG
Applications
WB, IHC-P, ICC
Purity
Antigen affinity
Format
Antigen affinity purified
Buffer
Lyophilized from 1X PBS with 2.5% BSA and 0.025% sodium azide/thimerosal
Reconstitution
Limitations
This NOX5 antibody is available for research use only.
Storage Conditions
Product Datasheet
https://www.nsjbio.com/tds-pdf/nox5-antibody-r31049
Formulation
0.5 mg/mL if reconstituted with 0.2ml sterile DI water
Applications Notes
Image Legend
Western blot testing of NOX5 antibody and Lane 1: 22RV1; 2: PANC; 3: HeLa; 4: SKOV. Expected/observed molecular weight: ~86 kDa.
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