Bub3 Antibody
Budding uninhibited by benzimidazoles 3 is a protein that in humans is encoded by the BUB3 gene. It is a protein involved with the regulation of the Spindle Assembly Checkpoint (SAC) ; though Bub3 is non-essential in yeast, it is essential in higher eukaryotes. As one of the checkpoint proteins, it delays the irreversible onset of anaphase through direction of kinetochore localization during prometaphase to achieve biorentation. Bub3 directs the localization of Bub1 at the kinetochore in order to activate the SAC. It also acts as a regulator in that it affects binding of Mad3 to Mad2.
Product Specifications
CAS Number
9007-83-4
Specifications
Western blot: 0.5-1 µg/mL, IHC (FFPE) : 0.5-1 µg/mL, IHC (Frozen) : 0.5-1 µg/mL, Immunocytochemistry: 0.5-1 µg/mL
UniProt
O43684
Host
Rabbit
Reactivity
Human, Mouse, Rat
Immunogen
Clonality
Polyclonal
Isotype
IgG
Applications
WB, IHC-P, IHC-F, ICC
Purity
Antigen affinity
Format
Antigen affinity purified
Buffer
Lyophilized from 1X PBS with 2.5% BSA and 0.025% sodium azide/thimerosal
Reconstitution
Limitations
This Bub3 antibody is available for research use only.
Storage Conditions
Product Datasheet
https://www.nsjbio.com/tds-pdf/bub3-antibody-r30729
Formulation
0.5 mg/mL if reconstituted with 0.2ml sterile DI water
Applications Notes
Image Legend
Western blot testing of Bub3 antibody and Lane 1: HeLa; 2: A549; 3: Jurkat; 4: COLO320 cell lysate
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