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BCAT2 Antibody / PP18 / Placental protein 18

Branched-chain aminotransferase 2, also called Placental protein 18, is a branched chain aminotransferase found in mitochondria. The encoded protein forms a dimer that catalyzes the first step in the production of the branched chain amino acids leucine, isoleucine, and valine. Multiple transcript variants encoding different isoforms have been found for this gene. The human BCAT2 gene is mapped to 19q13.

Product Specifications

CAS Number

9007-83-4

Specifications

Western blot: 0.5-1 µg/mL, IHC (FFPE) : 0.5-1 µg/mL

UniProt

O15382

Host

Rabbit

Reactivity

Human

Immunogen

An amino acid sequence from the C-terminus of human BCAT2 (KEIQYGIRAHEWMFPV) was used as the immunogen for this BCAT2 antibody.

Clonality

Polyclonal

Isotype

IgG

Applications

WB, IHC-P

Purity

Antigen affinity

Format

Antigen affinity purified

Buffer

Lyophilized from 1X PBS with 2.5% BSA and 0.025% sodium azide/thimerosal

Reconstitution

After reconstitution, the BCAT2 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Limitations

This BCAT2 antibody is available for research use only.

Storage Conditions

After reconstitution, the BCAT2 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Formulation

0.5 mg/mL if reconstituted with 0.2ml sterile DI water

Applications Notes

The stated application concentrations are suggested starting amounts. Titration of the BCAT2 antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Image Legend

Western blot testing of BCAT2 antibody and Lane 1: A431; 2: SW620; 3: SMMC-7721; 4: Jurkat; 5: CEM cell lysate

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