Apoptosis inhibitor 5 Antibody / API5

Many growth factors and cytokines act as cellular survival factors by preventing programmed cell death (apoptosis) . Apoptosis inhibitor 5 (API5) is an antiapoptotic factor which may have a role in protein assembly. The API5 gene to chromosome 11p12 based on an alignment of the API5 sequence with the genomic sequence It is a critical determinant of E2F1-induced apoptosis, acting downstream of E2F to suppress E2F-dependent apoptosis without generally blocking E2F-dependent transcription.

Product Specifications

CAS Number

9007-83-4

Specifications

Western blot: 0.5-1 µg/mL, Immunohistochemistry (FFPE) : 0.5-1 µg/mL, Immunohistochemistry (Frozen) : 0.5-1 µg/mL, Immunofluorescence: 5 µg/mL

UniProt

Q9BZZ5

Host

Rabbit

Reactivity

Human, Mouse, Rat

Immunogen

An amino acid sequence from the C-terminus of human Apoptosis inhibitor 5 (amino acids 487-504 of isoform b, KYSSNLGNFNYERSLQGK) was used as the immunogen for this Apoptosis inhibitor 5 antibody.

Clonality

Polyclonal

Isotype

IgG

Applications

WB, IHC-P, IHC-F, IF

Purity

Antigen affinity

Format

Antigen affinity purified

Buffer

Lyophilized from 1X PBS with 2.5% BSA and 0.025% sodium azide/thimerosal

Reconstitution

After reconstitution, the Apoptosis inhibitor 5 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Limitations

This Apoptosis inhibitor 5 antibody is available for research use only.

Storage Conditions

After reconstitution, the Apoptosis inhibitor 5 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Formulation

0.5 mg/mL if reconstituted with 0.2ml sterile DI water

Applications Notes

The stated application concentrations are suggested starting amounts. Titration of the Apoptosis inhibitor 5 antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Location

Nuclear, cytoplasmic

Image Legend

IHC staining of FFPE human placental tissue with Apoptosis inhibitor 5 antibody. HIER: boil tissue sections in pH6 citrate buffer for 20 min and allow to cool before testing.