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Myosin Antibody (Skeletal Fast)

Myosin is composed of 2 heavy chains of about 200 kD each and 4 light chains of about 20,000 daltons each. The light chains are of 2 distinct types: the phosphorylatable, regulatory, or MLC2 type, and the nonphosphorylatable, alkali, or MLC1 and MLC3 types. Skeletal Myosin (fast), also known as MYL1, mapped to the region 2q32.1-qter.

Product Specifications

CAS Number

9007-83-4

Specifications

Western blot: 1-2 µg/mL, IHC (FFPE) : 1-2 µg/mL

Host

Mouse

Reactivity

Human, Mouse, Rat, Rabbit

Immunogen

Rabbit muscle myosin was used as the immunogen for this Myosin antibody.

Clonality

Monoclonal

Isotype

IgG1

Clone

MY-32

Applications

WB, IHC-P

Purity

Ascites

Format

Ascites

Buffer

0.5mg/ml with 1% BSA and 0.01% sodium azide if reconstituted with 0.2ml sterile 1XPBS

Reconstitution

After reconstitution, the Myosin antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Limitations

This Myosin antibody is available for research use only.

Storage Conditions

After reconstitution, the Myosin antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Formulation

0.5 mg/mL with 1% BSA and 0.01% sodium azide if reconstituted with 0.2ml sterile 1X PBS

Applications Notes

The stated application concentrations are suggested starting amounts. Titration of the Myosin antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Image Legend

Western blot testing of Myosin antibody and rat skeletal muscle tissue lysate

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