Calbindin Antibody / CALB1

The human calbindin D28k (CALB1) is a calcium-binding protein belonging to the troponin C superfamily. It was originally described as a 27-kD protein induced by vitamin D in the duodenum of the chick. Parmentier et al. (1991) mapped the CALB1 gene to 8q21.3-q22.1 by in situ hybridization.

Product Specifications

CAS Number

9007-83-4

Specifications

Western blot: 0.5-1 µg/mL, IHC (FFPE) : 1-2 µg/mL, Immunofluorescence: 2-4 µg/mL

Host

Mouse

Reactivity

Human, Mouse, Rat, Bovine

Immunogen

Bovine kidney calbindin was used as the immunogen for this Calbindin antibody.

Clonality

Monoclonal

Isotype

IgG1

Clone

CB-955

Applications

WB, IHC-P, IF

Format

Ascites

Buffer

0.5mg/ml with 1% BSA and 0.01% sodium azide if reconstituted with 0.2ml sterile 1XPBS

Reconstitution

The lyophilized Calbindin antibody can be stored at 4oC to -20oC. After reconstitution, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Limitations

This Calbindin antibody is available for research use only.

Storage Conditions

The lyophilized Calbindin antibody can be stored at 4°C to -20°C. After reconstitution, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Formulation

0.5 mg/mL with 1% BSA and 0.01% sodium azide if reconstituted with 0.2ml sterile 1X PBS

Applications Notes

Titration of the Calbindin antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Location

Cytoplasmic

Image Legend

Western blot testing of Calbindin antibody and Lane 1: rat brain; 2: rat brain; 3: rat kidney; 4: rat lung tissue lysate. Expected molecular weight: 27-28 kDa.