Recombinant His-Tag Chimeric Human Antibody

This antibody is a Human-Rabbit chimeric antibody made by fusion of the antigen binding region (variable domains of the heavy and light chains, VH and VL) of rabbit anti-His Tag monoclonal antibody (RM146), with the constant domain of Human IgG1. The mAb is recognized only by an anti-Human IgG secondary antibody.

Product Specifications

CAS Number

9000-83-3

Specifications

ELISA (Capture) : 0. 1 µg/mL-2 µg/mL, ELISA (Detect) : 0.01 µg/mL-0.5 µg/mL, Western Blot: 0.1 µg/mL-1 µg/mL

Host

Rabbit

Immunogen

A mixture of a peptides with 6xHis-Tag at the N-terminus and peptides with 6xHis-Tag at the C-terminus was used as the immunogen for the recombinant His-Tag Chimeric Human antibody.

Clonality

Recombinant Monoclonal

Isotype

IgG

Clone

RMH01

Type

Recombinant

Applications

WB, ELISA

Purity

Protein A purified from animal origin-free supernatant

Format

Purified

Buffer

1 mg/ml in PBS with 50% glycerol, 1% BSA and 0.09% sodium azide

Reconstitution

Store the recombinant His-Tag Chimeric Human antibody at -20oC.

Limitations

This recombinant His-Tag Chimeric Human antibody is available for research use only.

Storage Conditions

Store the recombinant His-Tag Chimeric Human antibody at -20°C.

Formulation

1 mg/mL in PBS with 50% glycerol, 1% BSA and 0.09% sodium azide

Applications Notes

The stated application concentrations are suggested starting points. Titration of the recombinant His-Tag Chimeric Human antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Image Legend

Western blot testing of recombinant His-Tag Chimeric Human antibody (0.2ug/ml) with lysate from HEK293 cells transfected (+) or untransfected (-) with a His-Tag Histone H3 DNA construct. An anti-Human IgG secondary mAb was used as detect.