DDX4 Antibody
DEAD box proteins are RNA helicases involved in cellular processes involving the alteration of RNA secondary structure. DDX4, DEAD box polypeptide 4, is the mammalian homolog of the Drosophila VASA gene. DDX4 is expressed solely in ovarian and testicular germ cells and therefore a useful germ cell lineage marker and possible useful biomarker for germ cell cancers.
Product Specifications
CAS Number
9007-83-4
Specifications
Western blot: 2-5 µg/mL
Host
Rabbit
Reactivity
Human, Mouse
Immunogen
A recombinant protein fragment from the C-terminal region of DDX4/VASA was used as the immunogen for the DDX4 antibody.
Clonality
Polyclonal
Isotype
IgG
Applications
WB
Purity
Protein A affinity chromatography
Format
Purified
Buffer
1X PBS, pH 7.4
Reconstitution
Aliquot the DDX4 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.
Limitations
This DDX4 antibody is available for research use only.
Storage Conditions
Aliquot the DDX4 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.
Formulation
0.5 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced), 0.05% sodium azide
Applications Notes
Provided assay concentrations are suggestions only, antibody titration may be required for optimal results.
Image Legend
Western blot testing of human samples with DDX4 antibody at 2ug/ml.
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