UVRAG Antibody

UVRAG complements the ultraviolet sensitivity of xeroderma pigmentosum group C cells and encodes a protein with a C2 domain. The protein activates the Beclin1-PI (3) KC3 complex, promoting autophagy and suppressing the proliferation and tumorigenicity of human colon cancer cells. Chromosomal aberrations involving this gene are associated with left-right axis malformation and mutations in this gene have been associated with colon cancer.

Product Specifications

CAS Number

9007-83-4

Specifications

Immunofluorescence: 1:25, Immunohistochemistry (FFPE) : 1:25, Western blot: 1:500-1:2000

UniProt

Q9P2Y5

Host

Rabbit

Reactivity

Human, Mouse

Immunogen

A portion of amino acids 666-699 from the human protein was used as the immunogen for the UVRAG antibody.

Clonality

Polyclonal

Isotype

Applications

IF, IHC-P, WB

Purity

Purified

Format

Purified

Buffer

In 1X PBS, pH 7.4, with 0.09% sodium azide

Reconstitution

Aliquot the UVRAG antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Limitations

This UVRAG antibody is available for research use only.

Storage Conditions

Aliquot the UVRAG antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

Formulation

In 1X PBS, pH 7.4, with 0.09% sodium azide

Applications Notes

The stated application concentrations are suggested starting points. Titration of the UVRAG antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Image Legend

Immunoprecipitation and western blot analysis using UVRAG antibody with 293T cells. UVRAG is immunoprecipitated (Lane 2) and detected in 293T cell transiently transfected with mouse UVRAG (Lane 1) . Detection of endogenous UVRAG is shown in 293T cells (Lane 3) but is reduced by UVRAG siRNA transfection (Lane 4) . Data courtesy of Dr. Hong-Gang Wang, Moffitt Cancer Center, Tampa, FL.