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Anti-Neurofilament M (NF-M)

Product Specifications

CAS Number

9007-83-4

Gene Aliases

Nfm, Nef3

Gene ID

24588

Accession Number

NP_058725.1

Reactivity

Human, Mouse, Rat

Immunogen

Recombinant fusion protein containing the extreme C-terminus of rat NF-M expressed in and purified from E. coli.

Target

Neurofilaments are the 10nm or intermediate filament proteins found specifically in neurons, and are composed predominantly of three major proteins called NF-L, NF-M and NF-H (1) . NF-M is the neurofilament middle or medium molecular weight polypeptide and runs on SDS-PAGE gels at 145-160 kDa, with some variability across species boundaries. Antibodies to NF-M are useful for identifying neuronal cells and their processes in tissue sections and in tissue culture. NF-M antibodies can also be useful to visualize neurofilament accumulations seen in many neurological diseases, such as Amyotrophic Lateral Sclerosis (Lou Gehrig's disease) and Alzheimer's disease (2) .

Clonality

Polyclonal

Type

Polyclonal Antibody

Applications

WB, IF

Purification

Total IgY fraction.

Assay Protocol

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

Homology

Human; Mouse; Rat

Format

Liquid containing 10 mM sodium azide.

Reconstitution

Store at -20C in undiluted aliquots; stable for at least 1 year. Avoid freeze/thaw cycles.

Molecular Weight

~145kDa

Specificity

Specific for the ~145k Neurofilament M protein.

NCBI Gene Symbol

NEFM

Host or Source

Chicken

Protein Name

Neurofilament medium polypeptide

Gene Name URL

NEFM

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