Anti-Neurofilament M (NF-M)
Product Specifications
CAS Number
9007-83-4
Gene Aliases
Nfm, Nef3
Gene ID
24588
Accession Number
NP_058725.1
Reactivity
Human, Mouse, Rat
Immunogen
Recombinant fusion protein containing the extreme C-terminus of rat NF-M expressed in and purified from E. coli.
Target
Neurofilaments are the 10nm or intermediate filament proteins found specifically in neurons, and are composed predominantly of three major proteins called NF-L, NF-M and NF-H (1) . NF-M is the neurofilament middle or medium molecular weight polypeptide and runs on SDS-PAGE gels at 145-160 kDa, with some variability across species boundaries. Antibodies to NF-M are useful for identifying neuronal cells and their processes in tissue sections and in tissue culture. NF-M antibodies can also be useful to visualize neurofilament accumulations seen in many neurological diseases, such as Amyotrophic Lateral Sclerosis (Lou Gehrig's disease) and Alzheimer's disease (2) .
Clonality
Polyclonal
Type
Polyclonal Antibody
Applications
WB, IF
Purification
Total IgY fraction.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions
Reconstitution & Storage Instructions
Western Blotting/Immunoblotting (WB/IB) Protocol
Western Blotting/Immunoblotting (WB/IB) Protocol
Immunohistochemistry (IHC) Protocol
Immunohistochemistry (IHC) Protocol
Immunocytochemistry (ICC) Protocol
Immunocytochemistry (ICC) Protocol
Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
Blocking Peptide Competition Protocol (BPCP)
Blocking Peptide Competition Protocol (BPCP)
Immunoprecipitation (IP) Protocol
Immunoprecipitation (IP) Protocol
Antibody Array (AA) Protocol
Antibody Array (AA) Protocol
Homology
Human; Mouse; Rat
Format
Liquid containing 10 mM sodium azide.
Reconstitution
Store at -20C in undiluted aliquots; stable for at least 1 year. Avoid freeze/thaw cycles.
Molecular Weight
~145kDa
Specificity
Specific for the ~145k Neurofilament M protein.
NCBI Gene Symbol
NEFM
Host or Source
Chicken
Protein Name
Neurofilament medium polypeptide
Gene Name URL
NEFM
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