EFL1 Antibody / EFTUD1

Involved in the biogenesis of the 60S ribosomal subunit and translational activation of ribosomes. Together with SBDS, triggers the GTP-dependent release of EIF6 from 60S pre-ribosomes in the cytoplasm, thereby activating ribosomes for translation competence by allowing 80S ribosome assembly and facilitating EIF6 recycling to the nucleus, where it is required for 60S rRNA processing and nuclear export. Has low intrinsic GTPase activity. GTPase activity is increased by contact with 60S ribosome subunits. [UniProt]

Product Specifications

CAS Number

9007-83-4

Specifications

Immunohistochemistry (FFPE) : 1:25, Flow cytometry: 1:25 (1x10e6 cells), Western blot: 1:500-1:2000

UniProt

Q7Z2Z2

Host

Rabbit

Reactivity

Human, Mouse

Immunogen

A portion of amino acids 1062-1090 from the human protein was used as the immunogen for the EFL1 antibody.

Clonality

Polyclonal

Isotype

Applications

IHC-P, FACS, WB

Purity

Antigen affinity purified

Format

Purified

Buffer

In 1X PBS, pH 7.4, with 0.09% sodium azide

Reconstitution

Aliquot the EFL1 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Limitations

This EFL1 antibody is available for research use only.

Storage Conditions

Aliquot the EFL1 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

Formulation

In 1X PBS, pH 7.4, with 0.09% sodium azide

Applications Notes

The stated application concentrations are suggested starting points. Titration of the EFL1 antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Image Legend

IHC testing of FFPE human skeletal muscle tissue with EFL1 antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.