HLA-G Antibody
HLA-G belongs to the HLA class I heavy chain paralogues. This class I molecule is a heterodimer consisting of a heavy chain and a light chain (beta-2 microglobulin) . The heavy chain is anchored in the membrane. HLA-G is expressed on fetal derived placental cells. The heavy chain is approximately 45 kDa and its gene contains 8 exons. Exon one encodes the leader peptide, exons 2 and 3 encode the alpha1 and alpha2 domain, which both bind the peptide, exon 4 encodes the alpha3 domain, exon 5 encodes the transmembrane region, and exon 6 encodes the cytoplasmic tail.
Product Specifications
CAS Number
9007-83-4
Specifications
Western blot: 1:1000, IHC (Paraffin) : 1:10-1:50, Flow Cytometry: 1:10-1:50
UniProt
P17693
Host
Rabbit
Reactivity
Human
Immunogen
A portion of amino acids 62-89 from the human protein was used as the immunogen for this HLA-G antibody.
Clonality
Polyclonal
Isotype
Ig
Applications
WB, IHC, FACS, ELISA
Purity
Antigen affinity
Format
Antigen affinity purified
Buffer
In 1X PBS, pH 7.4, with 0.09% sodium azide
Reconstitution
Aliquot the HLA-G antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.
Limitations
This HLA-G antibody is available for research use only.
Storage Conditions
Aliquot the HLA-G antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.
Formulation
In 1X PBS, pH 7.4, with 0.09% sodium azide
Applications Notes
Titration of the HLA-G antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Image Legend
Western blot analysis of HLA-G antibody and 293 cell lysate either nontransfected (Lane 1) or transiently transfected (2) with the HLA-G gene.
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