Caspase-9 Antibody
Caspase 9 is a member of the cysteine-aspartic acid protease (caspase) family. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. Caspases exist as inactive proenzymes which undergo proteolytic processing at conserved aspartic residues to produce 2 subunits, large and small, that dimerize to form the active enzyme. This protein is processed by caspase APAF1; this step is thought to be one of the earliest in the caspase activation cascade.
Product Specifications
CAS Number
9007-83-4
Specifications
Western blot: 1:1000, IHC (Paraffin) : 1:10-1:50, Immunofluorescence: 1:10-1:50
UniProt
P55211
Host
Rabbit
Reactivity
Human, Mouse
Immunogen
A portion of amino acids 183-211 from the human protein was used as the immunogen for this Caspase-9 antibody.
Clonality
Polyclonal
Isotype
Ig
Applications
IHC, IF, WB, ELISA
Purity
Purified
Format
Purified
Buffer
In 1X PBS pH 7.4 with 0.09% sodium azide
Reconstitution
Aliquot the Caspase-9 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.
Limitations
This Caspase-9 antibody is available for research use only.
Storage Conditions
Aliquot the Caspase-9 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.
Formulation
In 1X PBS, pH 7.4, with 0.09% sodium azide
Applications Notes
Titration of the Caspase-9 antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Image Legend
Caspase-9 antibody western blot analysis in 1) human HeLa and 2) mouse L929 lysate
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