LCK Antibody

LCK, which belongs to the SRC subfamily of Tyr protein kinases, may participate in antigen-induced T-cell activation. It binds to phosphatidylinositol 3'-kinase (PI3K) from T lymphocytes through its SH3 domain and to the tyrosine phosphorylated form of KHDRBS1/p70 through its SH2 domain. LCK is bound to the cytoplasmic domain of either CD4 or CD8. This protein is involved in leukemias by a chromosomal translocation t (1;7) (p34; q34) which involves LCK and T-cell receptor beta chain (TCRB) genes.

Product Specifications

CAS Number

9007-83-4

Specifications

Western blot: 1:1000, IHC (Paraffin) : 1:50-1:100, Immunofluorescence: 1:10-1:50, Flow Cytometry: 1:10-1:50

UniProt

P06239

Host

Rabbit

Reactivity

Human, Mouse, Rat

Immunogen

A portion of amino acids 23-52 from the human protein was used as the immunogen for this LCK antibody.

Clonality

Polyclonal

Isotype

Applications

IHC, IF, FACS, WB, ELISA

Purity

Antigen affinity

Format

Antigen affinity purified

Buffer

In 1X PBS, pH 7.4, with 0.09% sodium azide

Reconstitution

Aliquot the LCK antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Limitations

This LCK antibody is available for research use only.

Storage Conditions

Aliquot the LCK antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

Formulation

In 1X PBS, pH 7.4, with 0.09% sodium azide

Applications Notes

Titration of the LCK antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Image Legend

Confocal immunofluorescent analysis of LCK antibody with A2058 cells followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green) . Actin filaments have been labeled with Alexa Fluor 555 Phalloidin (red) . DAPI was used as a nuclear counterstain (blue) .