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Rab5 Antibody

Rab5 is required for the fusion of plasma membranes and early endosomes.

Product Specifications

CAS Number

9007-83-4

Specifications

Western blot: 1:1000, IHC (Paraffin) : 1:50-1:100, Immunofluorescence: 1:10-1:50, Flow Cytometry: 1:10-1:50

UniProt

P20339

Host

Rabbit

Reactivity

Human

Immunogen

A portion of amino acids 182-211 from human Rab5a was used as the immunogen for this Rab5 antibody.

Clonality

Polyclonal

Isotype

Ig

Applications

WB, IHC, IF, FACS, ELISA

Purity

Antigen affinity

Format

Antigen affinity purified

Buffer

In 1X PBS, pH 7.4, with 0.09% sodium azide

Reconstitution

Aliquot the Rab5 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Limitations

This Rab5 antibody is available for research use only.

Storage Conditions

Aliquot the Rab5 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

Formulation

In 1X PBS, pH 7.4, with 0.09% sodium azide

Applications Notes

Titration of the Rab5 antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Location

Cytoplasmic

Image Legend

Rab5 antibody flow cytometric analysis of HL-60 cells (right histogram) compared to a negative control (left histogram) . FITC-conjugated goat-anti-rabbit secondary Ab was used for the analysis.

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