OGT Antibody
O-linked N-acetylglucosamine (O-GlcNAc) transferase (OGT) catalyzes the addition of a single N-acetylglucosamine in O-glycosidic linkage to serine or threonine residues. Since both phosphorylation and glycosylation compete for similar serine or threonine residues, the two processes may compete for sites, or they may alter the substrate specificity of nearby sites by steric or electrostatic effects. The protein contains nine tetratricopeptide repeats and a putative bipartite nuclear localization signal.
Product Specifications
CAS Number
9007-83-4
Specifications
Western blot: 1:1000, IHC (Paraffin) : 1:50-1:100
UniProt
O15294
Host
Rabbit
Reactivity
Human, Mouse
Immunogen
A portion of amino acids 1017-1046 from the human protein was used as the immunogen for this OGT antibody.
Clonality
Polyclonal
Isotype
Ig
Applications
WB, IHC, ELISA
Purity
Purified
Format
Purified
Buffer
In 1X PBS, pH 7.4, with 0.09% sodium azide
Reconstitution
Aliquot the OGT antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.
Limitations
This OGT antibody is available for research use only.
Storage Conditions
Aliquot the OGT antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.
Formulation
In 1X PBS, pH 7.4, with 0.09% sodium azide
Applications Notes
Titration of the OGT antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Prediction Reactivity
Rat, Pig, Rabbit
Location
Nuclear, cytoplasmic
Image Legend
Western blot analysis of OGT antibody and mouse bladder tissue lysate. Expected/observed molecular weight: 110-117 kDa.
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