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Phospho-eNos Antibody / NOS3 (pS1177)

ENos produces nitric oxide, a reactive free radical which acts as a biologic mediator in several processes, including neurotransmission and antimicrobial and antitumoral activities. Nitric oxide is synthesized from L-arginine by nitric oxide synthases.

Product Specifications

CAS Number

9007-83-4

Specifications

Dot blot: 1:500, Immunofluorescence: 1:10-1:50

UniProt

P29474

Host

Rabbit

Reactivity

Human

Immunogen

This phospho-eNos antibody was produced from rabbits immunized with a KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding pS1177 of human eNos.

Clonality

Polyclonal

Isotype

Ig

Applications

DB, IF, ELISA

Purity

Antigen affinity

Format

Antigen affinity purified

Buffer

In 1X PBS pH 7.4 with 0.09% sodium azide

Reconstitution

Aliquot the phospho-eNos antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Limitations

This phospho-eNos antibody is available for research use only.

Storage Conditions

Aliquot the phospho-eNos antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

Formulation

In 1X PBS, pH 7.4, with 0.09% sodium azide

Applications Notes

Titration of the phospho-eNos antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Prediction Reactivity

Bovine, Mouse, Pig, Rat

Image Legend

Dot blot analysis of phospho-eNos antibody. 50ng of phos-peptide or nonphos-peptide per dot were spotted.

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