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MeCP2 Antibody

DNA methylation is the major modification of eukaryotic genomes and plays an essential role in mammalian development. Human proteins MECP2, MBD1, MBD2, MBD3, and MBD4 comprise a family of nuclear proteins related by the presence in each of a methyl-CpG binding domain (MBD) . In contrast to other MBD family members, MECP2 is X-linked and subject to X inactivation. MECP2 is dispensible in stem cells, but is essential for embryonic development. [RefSeq].

Product Specifications

CAS Number

9007-83-4

Specifications

Western blot: 1:1000, Flow Cytometry: 1:10-1:50

UniProt

Q95LG8

Host

Rabbit

Reactivity

Human

Immunogen

A portion of amino acids 399-428 from the macfa protein was used as the immunogen for this MeCP2 antibody.

Clonality

Polyclonal

Isotype

Ig

Applications

WB, FACS, ELISA

Purity

Purified

Format

Purified

Buffer

In 1X PBS, pH 7.4, with 0.09% sodium azide

Reconstitution

Store at 4oC for up to one month. For long term, aliquot the MeCP2 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Limitations

This MeCP2 antibody is available for research use only.

Storage Conditions

Store at 4°C for up to one month. For long term, aliquot the MeCP2 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

Formulation

In 1X PBS, pH 7.4, with 0.09% sodium azide

Applications Notes

The stated application concentrations are suggested starting amounts. Titration of the MeCP2 antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Prediction Reactivity

Mouse

Image Legend

Western blot analysis of MeCP2 antibody and 293 cell lysate (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (2) with the MeCP2 gene. Observed molecular weight: ~55 kDa and ~75 kDa.

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