Alpha Actin Antibody

The product encoded by this gene belongs to the actin family of proteins, which are highly conserved proteins that play a role in cell motility, structure and integrity. Alpha, beta and gamma actin isoforms have been identified, with a-actins being a major constituent of the contractile apparatus, while beta and gamma actins are involved in the regulation of cell motility. This actin is an a-actin that is found in skeletal muscle. Mutations in this gene cause nemaline myopathy type 3, congenital myopathy with excess of thin myofilaments, congenital myopathy with cores, and congenital myopathy with fiber-type disproportion, diseases that lead to muscle fiber defects.

Product Specifications

CAS Number

9007-83-4

Specifications

Western blot: 1:1000, IHC (Paraffin) : 1:10-1:50, Immunofluorescence: 1:10-1:50, Flow Cytometry: 1:10-1:50

UniProt

P68133

Host

Rabbit

Reactivity

Human

Immunogen

A portion of amino acids 346-375 from human ACTA1 was used as the immunogen for this alpha Actin antibody.

Clonality

Polyclonal

Isotype

Applications

IHC, IF, FACS, WB, ELISA

Purity

Antigen affinity

Format

Antigen affinity purified

Buffer

In 1X PBS, pH 7.4, with 0.09% sodium azide

Reconstitution

Aliquot the alpha Actin antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Limitations

This alpha Actin antibody is available for research use only.

Storage Conditions

Aliquot the alpha Actin antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

Formulation

In 1X PBS, pH 7.4, with 0.09% sodium azide

Applications Notes

The stated application concentrations are suggested starting amounts. Titration of the alpha Actin antibody may be required due to differences in protocols and secondary/substrate sensitivity.