IRF4 Antibody

The protein encoded by this gene belongs to the IRF (interferon regulatory factor) family of transcription factors, characterized by an unique tryptophan pentad repeat DNA-binding domain. The IRFs are important in the regulation of interferons in response to infection by virus, and in the regulation of interferon-inducible genes. This family member is lymphocyte specific and negatively regulates Toll-like-receptor (TLR) signaling that is central to the activation of innate and adaptive immune systems. A chromosomal translocation involving this gene and the IgH locus, t (6;14) (p25; q32), may be a cause of multiple myeloma. Alternatively spliced transcript variants have been found for this gene.

Product Specifications

CAS Number

9007-83-4

Specifications

Western blot: 1:1000, Flow Cytometry: 1:10-1:50

UniProt

Q15306

Host

Rabbit

Reactivity

Human

Immunogen

A portion of amino acids 164-191 from the human protein was used as the immunogen for this IRF4 antibody.

Clonality

Polyclonal

Isotype

Applications

WB, FACS, ELISA

Purity

Purified

Format

Purified

Buffer

In 1X PBS, pH 7.4, with 0.09% sodium azide

Reconstitution

Aliquot the IRF4 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Limitations

This IRF4 antibody is available for research use only.

Storage Conditions

Aliquot the IRF4 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

Formulation

In 1X PBS, pH 7.4, with 0.09% sodium azide

Applications Notes

Titration of the IRF4 antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Image Legend

Western blot analysis of IRF4 antibody and 293 cell lysate (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (2) with the IRF4 gene. Predicted molecular weight: 51 kDa