BDNF Antibody

Product Specifications

Product Name Alternative

BDNF, ANON2, Abrineurin, BULN2, Neurotrophin

Reactivity

Bovine, Canine, Equine, Guinea pig, Hamster, Human, Monkey, Mouse, Porcine, Rat

Immunogen

Peptide with sequence ETKCNPMGYTKE, from the internal region of the protein sequence according to NP_001700.2NP_001700.2NP_733928.1NP_733929.1NP_733930.1NP_733931.1.

Target

BDNF

Clonality

Polyclonal

Conjugation

Unconjugated

Field of Research

Neuroscience

Purification

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Concentration

0.5 mg/ml

Dilution

IF (10 μg/ml), IHC, IHC-P (3.75 μg/ml), Peptide-ELISA (1:2000)

Storage Conditions

Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

Notes

For research use only.

Applications Notes

Further information: Immunohistochemistry: This was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration was determined to be 3.75ug/ml.