Insulin Antibody
Insulin Antibody
Product Specifications
Product Name Alternative
INS, IRDN, IDDM2, Preproinsulin, ILPR, Insulin, MODY10, Proinsulin
Reactivity
Human
Immunogen
This protein A purified monoclonal antibody was produced by repeated immunizations with purified human insulin coupled to bovine serum albumin (BSA) .
Target
Insulin
Clonality
Monoclonal
Clone
2D11.H5
Conjugation
Unconjugated
Field of Research
Metabolism Research
Purification
Protein A purified
Concentration
1 mg/ml
Dilution
DB, ELISA (1:5000 - 1:25000), IHC, IHC-P (5 μg/ml), IP, WB (1:50 - 1:200)
Storage Conditions
Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Notes
For research use only.
Applications Notes
Further information: Immunohistochemistry: This was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration was determined to be 5 ug/ml.
Tested Applications
DOT, ELISA, IHC, IHC-P, IP, WB
Host or Source
Mouse
Preservative
0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide
Isotype
IgG1, k
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