Anti-BAD (Phospho-Ser112) Antibody

Rabbit Polyclonal Antibody specific to BAD (Phospho-Ser112)

Product Specifications

CAS Number

9007-83-4

Product Name Alternative

Anti-BAD (Phospho-Ser112) Rabbit Polyclonal Antibody

Gene Name

BAD

Gene ID

603167

NCBI Gene ID

UniProt

Q92934

Cellular Locus

Mitochondrion outer membrane. Cytoplasm

Host

Rabbit

Reactivity

Human, Mouse

Immunogen

Peptide sequence that includes phosphorylation site of Serine 112 (H-S-S(p)-Y-P) derived from mouse BAD and conjugated to KLH.

Target Antigen

Bcl2-associated agonist of cell death

Target

BAD (Phospho-Ser112)

Clonality

Polyclonal

Type

Antibody

Applications

WB, IHC

Field of Research

Phosphospecific Antibodies

Purification Method

Affinity-purified on phosphopeptide; non-phosphopeptidereactive antibodies were removed by chromatography on non-phosphorylated peptide

Concentration

1.0 mg/mL

Dilution

Dilute in PBS or medium that is identical to that used in the assay system.

Format

Purified

Form

Liquid

Buffer

Phosphate Buffered Saline

Function

Promotes cell death. Successfully competes for the binding to Bcl-X(L), Bcl-2 and Bcl-W, thereby affecting the level of heterodimerization of these proteins with BAX. Can reverse the death repressor activity of Bcl-X(L), but not that of Bcl-2 (By similarity). Appears to act as a link between growth factor receptor signaling and the apoptotic pathways. {ECO:0000250}.

Additionnal Information

Immunoblotting: use at dilution of 1:500- 1:1,000. A ban of ~23kDa is detected. Immunohistochemistry: use at dilution of 1:50- 1:100. These are recommended working dilutions. End user should determine optimal dilutions for their application.

Storage Conditions

This antibody is stable for at least one (1) year at -20°C.

Specificity

This antibody detects endogenous human and mouse BAD only when phosphorylated at serine 112.Accession no.: Q61337, NP_031548.1

Formulation

PBS (without Mg2 and Ca2 ), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Buffer pH

pH 7.4

Target Background

The protein encoded by the BAD gene is a member of the Bcl-2 family whose members are regulators of programmed cell death. BAD protein positively regulates cell apoptosis by forming heterodimers with Bcl-xL and Bcl-2 and reversing their death repressor activity. Proapoptotic activity of this protein is regulated through its phosphorylation. Protein kinases AKT and MAP kinase, as well as protein phosphatase calcineurin, were found to be involved in the regulation of this protein. Alternative splicing of this gene results in two transcript variants which encode the same isoform.