RELA Antibody
NFkB p65 antibody
Product Specifications
Product Name Alternative
Rabbit Anti-NFKB p65 antibody, rabbit Anti-Rel A antibody, NFKB, nfkb, NF-kB, NF-kappaB, NFkappaB, Nuclear factor NF-kappa-B p65 subunit
UniProt
Q04206
Reactivity
Human, Mouse
Immunogen
NFkB p65 (Rel A) peptide corresponding to a region near the C-terminus of the human protein conjugated to Keyhole Limpet Hemocyanin (KLH) .
Target
RELA
Clonality
Polyclonal
Conjugation
Unconjugated
Concentration
80 mg/mL
Dilution
ELISA: 1:5,000, ChIP: 1 μl/IP, Gel Supershift Assays: 05 μL - 1.0 μL, IHC: 1:400, WB: 1:2,000 - 1:5,000
Purity
This product was prepared from monospecific antiserum by delipidation and defibrination. Anti-NFkB p65 (Rel A) may react non-specifically with other proteins. Control peptide (code #100-4165p) will compete only with the specific reaction of antiserum with the NFkB p65 (Rel A) subunit.
Form
Liquid (sterile filtered)
Storage Conditions
Store vial at -20° C or below prior to opening. This vial contains a relatively low volume of reagent (25 μL) . To minimize loss of volume dilute 1:10 by adding 225 μL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below. Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.
Notes
For research use only.
Applications Notes
Anti-NFkB p65 (Rel A) is tested by immunoblot of human and mouse NFkB p65 (Rel A), immunohistochemistry, and ELISA. This product was also tested in a gel supershift assay, IP, IF, and ChIP and found to be reactive against all p65 (Rel A) containing human, mouse or rat NFkB complexes using 0.5 to 1.0 μl per assay.
Tested Applications
ChIP, ELISA, Gel Supershift Assays, IHC, WB
NCBI Accession Number
223468676
Host or Source
Rabbit
Preservative
0.01% (w/v) Sodium Azide
Isotype
Antiserum
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