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TLR4 Antibody

TLR4 antibody

Product Specifications

Product Name Alternative

Rabbit Anti-TLR4 Antibody, ARMD10 antibody, CD284 antibody, Toll (Drosophila) homolog antibody, HToll, Homolog of Drosophila Toll, TLR-4 Antibody, TOLL antibody, Toll Endotoxin Hyporesponsiveness antibody, Toll like receptor 4 antibody, Toll like receptor 4 precursor antibody

UniProt

O00206

Reactivity

Human

Immunogen

This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to a region near the n-terminus of human TLR4 protein.

Target

TLR4

Clonality

Polyclonal

Conjugation

Unconjugated

Field of Research

Immunology & Inflammation

Concentration

1.0 mg/ml

Dilution

ELISA: 5 ug/ml, IHC: 1:100 - 1:200, WB: 1:1000

Purity

This affinity purified antibody is directed against human TLR4 protein. The product was purified from monospecific antiserum by immunoaffinity chromatography. A BLAST analysis was used to suggest reactivity with TLR4 protein from human based on 100% homology with the immunizing sequence. The analysis also shows 100% cross-reactivity with gorilla and bonobo, and canine species.

Form

Liquid (sterile filtered)

Storage Conditions

Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Notes

For research use only.

Applications Notes

Anti-TLR4 affinity purified antibody has been tested for use in ELISA, Immunohistochemistry, and western blot. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 96 kDa in size corresponding to TLR4 protein in overexpressed lysate by western blotting in the appropriate cell lysate or extract.

Tested Applications

ELISA, IHC, WB

NCBI Accession Number

19924149

Host or Source

Rabbit

Preservative

Preservative: 0.01% (w/v) Sodium Azide. Stabilizer: None; Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Isotype

IgG

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