HIST2H3C Antibody

Rabbit anti-Histone H3 trimethyl Lys18 pS28 antibody, H3.3B, H3pS28K18Me3, H3 histone, family 3A, H3.3AH3F3H3F3B, histone H3.3, MGC87783, MGC87782, H3 K27me3/pS28

Histone H3 [Trimethyl Lys27, p Ser28] affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with synthetic trimethylated/phosphorylated peptides surrounding Lysine 27 and Serine 28 of human Histone H3.

Anti-Histone H3 [Trimethyl Lys27, p Ser28] was affinity purified from monospecific antiserum by immunoaffinity chromatography. This antibody reacts with human Histone H3. A BLAST analysis was used to suggest cross-reactivity with Human, mouse, and C. elegans. Predicted to react with many species including rat, chicken, Xenopus, Drosophila, and plant based on 100% sequence homology. Cross-reactivity with Histone H3 from other sources has not been determined.

Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Anti-Histone H3 [Trimethyl Lys27, p Ser28] antibody is tested for Western Blot, Chromatin Immunoprecipitation, Dot Blot, and Immunocytochemistry/Immunofluorescence. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately ~15.4 kDa corresponding to Histone H3 protein by Western Blotting in HeLa histone prep lysate or the appropriate cell lysate or extract. Epi-Plus antibody production in collaboration with Novus Biologicals.