Sheep IgG Fc Antibody
Sheep IgG F (c) antibody
Product Specifications
Product Name Alternative
Rabbit Anti-Sheep IgG F (c) Antibody, Rabbit Anti-Sheep IgG Fc Antibody, Rabbit Anti-Sheep IgG Fc Fragment Antibody
Reactivity
Sheep
Immunogen
Anti-Sheep IgG was produced by repeated immunization with sheep IgG F (c) fragment in rabbit.
Clonality
Polyclonal
Conjugation
Unconjugated
Concentration
2.0 mg/mL
Dilution
ELISA: 1:10,000 - 1:200,000, IHC: 1:500 - 1:2,500, WB: 1:1,000 - 1:5,000
Purity
Anti-Sheep IgG F (c) was prepared from monospecific antiserum by immunoaffinity chromatography using Sheep IgG coupled to agarose beads. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum, Sheep IgG, Sheep IgG F (c) and Sheep Serum. No reaction was observed against Sheep IgG F (ab) .
Form
Liquid (sterile filtered)
Storage Conditions
Store vial at 4° C prior to opening. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing.
Notes
For research use only.
Applications Notes
Anti-Sheep IgG F (c) antibody has been tested by ELISA and dot blot and is assayed against 1.0 ug of Sheep IgG in a standard capture ELISA using ABTS (2,2’-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes at room temperature. A working dilution of 1:2,000 to 1:8,000 of the reconstitution concentration is suggested for this product.
Tested Applications
DOT, ELISA, IHC, WB
Host or Source
Rabbit
Preservative
Preservative: 0.01% (w/v) Sodium Azide. Stabilizer: None; Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Isotype
IgG
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