DDB1 Antibody

Rabbit anti-Anti-p127-DDB1 antibody, Damage specific DNA binding protein 1 antibody, DDB 1 antibody, DDB p127 subunit antibody, DDBa antibody, DNA damage binding protein 1 antibody, UV damaged DNA binding factor antibody

DDB1 antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to amino acids 198-213 of Human DDB1 (internal) coupled to KLH.

Anti-DDB1 is monospecific antiserum processed by delipidation and defibrination followed by sterile filtration. This product reacts with human and mouse DDB1. Cross reactivity with DDB1 from other sources is not known.

Store Anti-DDB1 at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Anti-DDB1 antibody reacts with human and mouse DDB1 tested by western blot and immunoprecipitation. The antibody immunoprecipitates in vitro translated protein and protein from cell lysates (using HeLa, NIH-3T3, and others) . Coimmunoprecipitation of related proteins has not been tested. A 127.0 kDa band corresponding to human DDB1 is detected. Most cell lines expressing DDB1 can be used as a positive control. Researchers should determine optimal titers for other applications.