Human IgG (H&L) Antibody
Human IgG (H&L) antibody
Product Specifications
Product Name Alternative
Human Antibody, Rb-a-Human IgG, Human Antibody in Rabbit, Human Secondary Antibody
Reactivity
Human
Immunogen
Anti-Human IgG whole molecule was produced by repeated immunization with Human IgG whole molecule in rabbit.
Clonality
Polyclonal
Conjugation
Unconjugated
Concentration
10.0 mg/mL
Dilution
ELISA: 1:20,000 - 1:100,000, IHC: 1:1,000 - 1:5,000, WB: 1:2,000 - 1:10,000
Purity
This product is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum, Human IgG and Human Serum.
Form
Lyophilized
Storage Conditions
Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
Notes
For research use only.
Applications Notes
Anti-Human IgG antibody is suitable for immunoblotting (western or dot blot), ELISA, immunoelectron microscopy and immunohistochemistry as well as other antibody based enzymatic assays requiring lot-to-lot consistency.
Tested Applications
ELISA, IHC, WB
Host or Source
Rabbit
Preservative
Preservative: None. Stabilizer: None; Buffer: 0.01 M Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Isotype
IgG
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