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APP Antibody

Beta Amyloid protein 40 antibody

Product Specifications

Product Name Alternative

Rabbit anti-Beta Amyloid 40 Antibody, ß-amyloid 40, Amyloid beta A4 protein, Alzheimer disease amyloid protein, Beta amyloid, A-beta, ABPP, APPI

UniProt

P05067

Reactivity

Human, Mouse, Primate, Rat

Immunogen

Beta Amyloid 40 affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to the C-terminus region of AB40.

Target

APP

Clonality

Polyclonal

Conjugation

Unconjugated

Field of Research

Cell Biology

Concentration

1.12 mg/ml

Dilution

ELISA: 1:20,000 - 1:60,000, IHC: 1:100-1:500, IF: 1:100-1:500, WB: 1 μg/ml

Purity

Anti-Beta Amyloid 40 was affinity purified from monospecific antiserum by immunoaffinity chromatography. A BLAST analysis was used to suggest reactivity with human, sheep, rabbit, canine, bovine, pig, guinea pig, and monkey based on 100% sequence homology. This antibody will not react with AB42. Cross-reactivity with Beta Amyloid from other sources has not been determined.

Form

Liquid (sterile filtered)

Storage Conditions

Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Notes

For research use only.

Applications Notes

Anti-Beta Amyloid 40 antibody is useful for Western Blot and ELISA. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately ~86.9 kDa corresponding to the appropriate cell lysate or extract.

Tested Applications

ELISA, IF, IHC, WB

NCBI Accession Number

NP_000475.1

Host or Source

Rabbit

Preservative

Preservative: None. Stabilizer: 50% (v/v) Glycerol with 1 mg/ml Bovine Serum Albumin (rAlbumin) ; Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Isotype

IgG

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