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BIN1 Antibody

BIN1 antibody

Product Specifications

Product Name Alternative

AMPHL, Myc box-dependent-interacting protein 1, Amphiphysin II, Amphiphysin-like protein, Box-dependent myc-interacting protein 1, Bridging integrator 1, BIN 1, BIN-1, mouse anti-BIN1 antibody, anti-BIN1 antibody

UniProt

O00499

Reactivity

Human

Immunogen

Anti-BIN1 (MOUSE) Monoclonal Antibody was produced in mouse by repeated immunizations with 12A exon BIN1 protein followed by hybridoma development.

Target

BIN1

Clonality

Monoclonal

Clone

12A

Conjugation

Unconjugated

Field of Research

Stem Cell & Developmental Biology

Concentration

1.0 mg/mL

Dilution

ELISA: 1:5000 - 1:50000, WB: 1:500-1:1500

Purity

Anti-BIN1 purified from clarified mouse ascetic fluid by Protein A chromatography followed by extensive dialysis against the buffer stated above. This antibody is specific for human BIN1 protein. A BLAST analysis was used to suggest cross-reactivity with BIN1 from human sources based on 100% homology with the immunizing sequence. Cross-reactivity with BIN1 from other sources has not been determined.

Form

Liquid (sterile filtered)

Storage Conditions

Store vial at -20° C or below prior to opening. This vial contains a relatively low volume of reagent (25 μL) . To minimize loss of volume dilute 1:10 by adding 225 μL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below. Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.

Notes

For research use only.

Applications Notes

Anti-BIN1 antibody has been tested for use in ELISA and Western Blot. Specific conditions for reactivity should be optimized by the end user.

Tested Applications

ELISA, WB

NCBI Accession Number

NP_004296.1

Host or Source

Mouse

Preservative

Preservative: 0.01% (w/v) Sodium Azide. Stabilizer: None; Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Isotype

IgG

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