NDUFA9 Antibody / NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 9

ELISA: 0.1-0.5 µg/mL, Flow cytometry: 1-3ug/million cells, Immunofluorescence: 5 µg/mL, Immunohistochemistry: 2-5 µg/mL, Immunocytochemistry: 5 µg/mL, Western Blot: 0.25-0.5 µg/mL

After reconstitution, the NDUFA9 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

IHC analysis of NDUFA9 using anti-NDUFA9 antibody. NDUFA9 was detected in a paraffin-embedded section of human breast cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution) . The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 ug/ml rabbit anti-NDUFA9 antibody overnight at 4oC. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37oC. The tissue section was developed using an HRP secondary and DAB substrate.